FIGURE 3.

Induction mechanism of SULT1A1/3. A, SK-N-MC cells were co-treated with 20 μm MEK inhibitor SL327 (SL) and 100 μm dopamine (DA) for 24 h. Phospho-ERK (p-ERK) and total ERK1/2 (Total-ERK) protein levels were quantified by Western blotting. B, cells were co-treated with 20 μm MEK inhibitor SL327 and 100 μm dopamine for 24 h. SULT1A1/3 protein levels were quantified by Western blotting. C, effect of norepinephrine and dibutyryl cAMP on ERK1/2 phosphorylation is shown. Cells were treated with 100 μm dopamine, 50 μm norepinephrine (NA), or 500 μm dibutyryl cAMP for 24 h. Phospho-ERK and total ERK1/2 protein levels were quantified by Western blotting. D, cells were treated with 100 μm dopamine, 50 μm norepinephrine, or 500 μm dibutyryl cAMP for 24 h. SULT1A1/3 protein levels were quantified by Western blotting. All results are the mean ± S.E. (error bars), n = 3, normalized to tubulin as loading control and are expressed as percentage of control (C). Asterisks indicate data significantly different (p < 0.05) from control by Student's t test.