FIGURE 6.

Effect of SULT1A1/3 induction on dopamine toxicity. A, SK-N-MC cells co-treated with various inhibitors and 100 μm dopamine (DA) for 24 h. Cell viability was then determined. B, dose-response curve for the 20 μm MEK inhibitor SL327. SK-N-MC cells were treated with inhibitor and increasing concentrations of dopamine for 24 h following which cell viability was determined. C, effect of various inhibitors on induction of SULT1A1/3 by 10 μm dopamine. SK-N-MC cells were co-treated with various inhibitors and 10 μm dopamine for 24 h. Representative Western blots are shown from three independent experiments. C, control; DA, dopamine; SCH, SCH23390; SL, SL327; Ket, ketamine. All results are the mean ± S.E. (error bars), n = 3, normalized to no dopamine treatment (control). Asterisks indicate data significantly different (p < 0.05) from control by Student's t test.