FIGURE 3.

Prohibitin is a direct target of miR-27a and miR-27b. A, schematic representation of hsa-miR-27a and hsa-miR-27b binding to the human PHB 3′-UTR. The seed sites of mature miR-27a and miR-27b are highlighted. The dashes indicate the six nucleotides (CTGTGA) that deleted in the mutant construct (Lenti/Luc-UTR/PHB^mut). B, ASC were transduced with Lenti/miR-Control (miR-Cont), Lenti/miR-27a (miR-27a), or Lenti/miR-27b (miR-27b) at m.o.i. = 2 and incubated overnight. ASC were then transduced with Lenti/Luc-UTR/Blank, Lenti/Luc-UTR/PHB, or Lenti/Luc-UTR/PHB^mut at m.o.i. = 1 as indicated. Following an additional 2 days of incubation, the luciferase activities in ASC were determined by luminometry. **, p < 0.01.