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. 2013 Oct 4;288(48):34612–34626. doi: 10.1074/jbc.M113.494369

FIGURE 4.

FIGURE 4.

Hemin inhibits Th17 cell differentiation in a dose-dependent manner in vitro. A, flow cytometric analysis of magnetically purified naïve T cells from spleens of BALB/c mice cultured under Th17-skewing conditions with or without different concentrations of hemin (10, 20, 30, 40 nmol/ml) for 3 days. Numbers in the upper right quadrants indicate the percentages of Th17 (CD4+ IL-17+) cells gated on CD4+ T cells (* compared with the control group: *, p < 0.05; #, with OVA group; #, p < 0.05). B, ELISA analysis of IL-17A in supernatants of cultured naïve T cells (* compared with the Th0 condition: *. p < 0.05; **, p < 0.01; ***, p < 0.001; # with the Th17-skewing condition: #, p < 0.05, ##, p < 0.01; ###, p < 0.001). All results shown are representative of three independent experiments.