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. 2013 Oct 4;288(48):34612–34626. doi: 10.1074/jbc.M113.494369

FIGURE 7.

FIGURE 7.

HO-1 does not noticeably affect Th1- or Th2-mediated response in DO11.10 mice. A, ELISA analysis of IFN-γ in BALF obtained from four groups. CON, control. B, ELISA analysis of IL-4 in BALF collected from four groups. C, flow cytometric analysis of mediastinal lymph node (LN) and spleen (SP) cells isolated from DO11.10 mice in control, OVA, OVA + hemin, and OVA + SnPP groups. Numbers in the upper left and lower right quadrants indicate the percentages of Th1 (CD4+IFN-γ+) cells and Th2 (CD4+IL-4+) cells gated on CD4+ T cells, respectively. Each symbol in the graph represents an individual mouse (n = 6). D, real-time PCR analysis of T-bet mRNAs in lung tissues from four groups. E, real-time PCR analysis of GATA-binding protein 3 mRNAs in lung tissues from four groups.