FIGURE 5.

Heterologously expressed gC1q-R in HEK 293 cells showed specific localization on the plasma membrane. HEK 293 cells were transfected with pDG1-MN1 vectors (expressing green fluorescence protein Dronpa) or rat gC1q-R cDNA cloned into pDG1-MN1 vectors (expressing gC1q-R/Dronpa chimeric protein). The cells transfected with control pDG1-MN1 vectors express Dronpa all over them. However, most of the gC1q-R/Dronpa chimeric proteins were located specifically in the plasma membrane as green fluorescent rings can be observed. The scale bars are all 50 μm.