FIGURE 4.

Time-resolved fluorescence of mBBr labeled W127C under rigor (orange), rigor + MT (blue), AMPPNP + MT (green), AMPPNP (magenta), and ADP-MT (red) biochemical states. A, time-resolved fluorescence detected at 470 ± 10 nm. The colored curves represent the data, and the gray curves represent the global TRF fit. B, mole fraction of lifetime species (τ₁ (blue), τ₂ (red), and τ₃ (green)) from the global TRF fit depicted in A. Statistical significance (p < 0.05) was determined by one-way analysis of variance. *, significant between a given lifetime mole fraction and the mole fraction of that lifetime in the rigor sample; ‡, significant difference between a given lifetime mole fraction and the mole fraction of that lifetime in the ADP sample. Error bars, S.E. C, global TRF emission spectra of lifetime species in B (τ₁ (blue), τ₂ (red), and τ₃ (green)). The peak for each emission spectrum is normalized to 1.0.