TABLE 3.

Time-resolved fluorescence rotational correlation times, final anisotropies, and cone angles as a function of biochemical state

Biochemical state	Correlation time
	τ1, 9.4 ± 0.07 ns	τ2, 1.3 ± 0.02 ns	τ3, 0.01 ± 0.02 ns
Final anisotropy (r∞)
Rigor	0.12 ± 0.01	0.08 ± 0.01	0 ± 0.01
Rigor + MTs	0.21 ± 0.01	0.07 ± 0.01	0 ± 0.01
AMPPNP	0.05 ± 0.004	0 ± 0.005	0 ± 0.01
AMPPNP + MTs	0.14 ± 0.004	0.04 ± 0.005	0 ± 0.01
ADP	0.03 ± 0.003	0 ± 0.004	0 ± 0.01
Semi-cone Angle (θ)
Rigor	48.6 ± 3.7	54.7 ± 6.9	90
Rigor + MTs	36.4 ± 1.6	56.8 ± 8.3	90
AMPPNP	59.9 ± 3.7	90	90
AMPPNP + MTs	45.8 ± 1.1	64.4 ± 7.7	90
ADP	66.9 ± 5.7	90	90