FIGURE 6.

Ca²⁺ accumulation and cell death in Ca²⁺ pathway mutants in response to commonly used antifungals. A, C, and E, ⁴⁵Ca²⁺ uptake into strains listed in Fig. 5 in SC-100 medium (10 mm KCl) after exposure to 0.25 μg/ml miconazole (MIC) (A), 25 μg/ml terbinafine (TB) (C), or 25 μg/ml caspofungin (CAS) (E) in the presence or absence of 1 μg/ml FK506 after 4 h of incubation. Averages for three biological replicates (±S.D.) are shown, and significant differences between kch1 and KCH1 control cells are indicated (**; see “Experimental Procedures”). B, D, and F, cell death measurements on saturated cultures in SC media in the presence of indicated concentrations of miconazole (B), terbinafine (D), or caspofungin (F) in the presence or absence of 1 μg/ml FK506 after a 24-h incubation. Cells were stained with propidium iodide and measured by flow cytometry.