FIGURE 4.

Inhibition of transient PRL effects with PKC isoform-specific inhibitors. A, pretreatment (5 min) with PRL (0.1 μg/ml) increased I_CAP (50 nm) in TG sensory neurons from female rats at both physiological concentrations of extracellular (2 mm) and intracellular (≈150 nm) Ca²⁺ and Ca²⁺-free (extracellular and intracellular) conditions. Unpaired t test was used (**, p < 0.01; n = 7–9; error bars, S.E.). Veh, vehicle. B, effect of PKCϵ (ϵ-V1-2 PKC, 1 μm), PKCδ (δPKC (8–17), 1 μm), and PKCζ (ζPKC(113–129) of PKCζ, 1 μm) inhibitors on sensitization of CAP (50 nm)-evoked Ca²⁺ influx by PRL (1 μg/ml) in TG sensory neurons from female rats is shown. Cells were pretreated for 10 min with inhibitors and then 5 min with inhibitors + PRL. CAP was applied for 1 min. Numbers of responding cells are indicated within bars. One-way ANOVA with Bonferroni's post hoc test was used (*, p < 0.05; ***, p < 0.001; NS, nonsignificant); error bars, S.E.