FIGURE 6.
A, enhancement of magnitude of ICAP (50 nm) by PRL (0.1 μg/ml) in CHO cells co-expressing GFP (for visualization of transfected cells), rat TRPV1, and rat PRLR-L or PRLR-S isoforms. Treatments by PRL were for 5 min in all experiments. CAP was applied for ≈40 s. Statistical analysis was unpaired t test (NS, nonsignificant; **, p < 0.01; n = 7–10; error bars, S.E.). B, representative ICAP traces from CHO cells transfected with GFP, TRPV1, and PRLR-S and pretreated with vehicle (Veh) or PRL. C, representative ICAP traces from CHO cells transfected with GFP, TRPV1, and PRLR-L, and pretreated with vehicle or PRL. Durations of CAP applications are indicated.