Table 1.
Proteins co-purifying with Tip60-H3F in ESCs
| Name | Description | # of peptides | MW (kD) | Peptides/MW | Gel slices |
|---|---|---|---|---|---|
| Stk38 | Serine/threonine-protein kinase 38 | 78 | 54 | 1.44 | 7,14,15,16,17 |
| Ruvbl1 | RuvB-like 1 | 42 | 50 | 0.84 | 7,8,13,15,16,17 |
| Ruvbl2 | RuvB-like 2 | 38 | 51 | 0.75 | 15,16,17 |
| Acta1 or other iso | Actin | 21 | 42 | 0.50 | 18 |
| Sun2 | Protein unc-84 homolog B | 36 | 82 | 0.44 | 11,12,13 |
| Hdac6 | Histone deacetylase 6 | 38 | 126 | 0.30 | 1,2,3,4,5,6,7,8 |
| Kat5 | Histone acetyltransferase KAT5 | 17 | 59 | 0.29 | 13,14,15 |
| Actb | Actin, cytoplasmic 1 | 12 | 42 | 0.29 | 5,8,9 |
| Trrap | Transformation/transcription domain-associated protein | 72 | 292 | 0.25 | 1,2 |
| Epc1 | Enhancer of polycomb homolog 1 | 22 | 90 | 0.24 | 9,10 |
| Brd8 | Bromodomain-containing protein 8 | 24 | 103 | 0.23 | 6,7 |
| Yeats4 | YEATS domain-containing protein 4 | 6 | 27 | 0.22 | 20 |
| Epc2 | Enhancer of polycomb homolog 2 | 20 | 91 | 0.22 | 10,11 |
| H2B | Histone H2B | 3 | 14 | 0.21 | 21 |
| Ing3 | Inhibitor of growth protein 3 | 10 | 47 | 0.21 | 16,17 |
| Ep400 | E1A-binding protein p400 | 70 | 337 | 0.21 | 1,2 |
| Dmap1 | DNA methyltransferase 1-associated protein 1 | 10 | 53 | 0.19 | 14,15 |
| Hspa8 | Heat shock cognate 71 kDa protein | 13 | 69 | 0.19 | 13 |
| Lima1 | LIM domain and actin-binding protein 1 | 15 | 84 | 0.18 | 9,10 |
| Vps72 | Vacuolar protein sorting-associated protein 72 homolog | 7 | 41 | 0.17 | 16,17 |
| Actl6a | Actin-like protein 6A | 8 | 47 | 0.17 | 17,18 |
| Actg1 | Actin, cytoplasmic 1 | 6 | 42 | 0.14 | 19 |
| H2afv | Histone H2A.V | 2 | 14 | 0.14 | 22 |
| Meaf6 | Chromatin modification-related protein MEAF6 | 3 | 22 | 0.14 | 20 |
| Mbtd1 | MBT domain-containing protein 1 | 8 | 71 | 0.11 | 13,14 |
| Rps18 | 40S ribosomal protein S18 | 2 | 18 | 0.11 | 21 |
| Tubb5 | Tubulin beta-5 chain | 5 | 50 | 0.10 | 16 |
| Tuba1a or other iso | Tubulin alpha chain | 5 | 50 | 0.10 | 16 |
| Trim28 | Transcription intermediary factor 1-beta | 8 | 89 | 0.09 | 9,10 |
| Morf4l2 | Mortality factor 4-like protein 2 | 3 | 34 | 0.09 | 19 |
| Mrgbp | MRG-binding protein | 2 | 24 | 0.08 | 20 |
| Rangap1 | Ran GTPase-activating protein 1 | 5 | 64 | 0.08 | 12 |
| Spna2 | Spectrin alpha chain, brain | 18 | 285 | 0.06 | 3 |
| Setx | Probable helicase senataxin | 18 | 298 | 0.06 | 2,3 |
| Sf3b1 | Splicing factor 3B subunit 1 | 3 | 54 | 0.06 | 6 |
| Sfpq | Splicing factor, proline- and glutamine-rich | 4 | 75 | 0.05 | 10 |
| Rab5c | Ras-related protein Rab-5C | 1 | 23 | 0.04 | 20 |
| Lrrfip2 | Leucine-rich repeat flightless-interacting protein 2 | 2 | 47 | 0.04 | 16 |
| Stat2 | Signal transducer and activator of transcription 2 | 2 | 50 | 0.04 | 8 |
| Nono | Non-POU domain-containing octamer-binding protein | 2 | 55 | 0.04 | 15 |
| Tpr | Nucleoprotein TPR | 6 | 274 | 0.02 | 3 |
| Hnrnpf | Heterogeneous nuclear ribonucleoprotein F | 1 | 46 | 0.02 | 17 |
| Spnb2 | Spectrin beta chain, brain 1 | 5 | 274 | 0.02 | 3 |
| Flna | Filamin-A | 4 | 281 | 0.01 | 3 |
| Flii | Protein flightless-1 homolog | 2 | 145 | 0.01 | 6 |
| Hdx | Highly divergent homeobox | 1 | 77 | 0.01 | 11 |
| Morf4l1 | Mortality factor 4-like protein 1 | 0 | 41 | 0 | N/A |
Proteins in bold represent known Tip60-p400 subunits found in Tip60-H3F purification from ESCs. The protein in bold italic represents the known Tip60-p400 subunit not found in purification from ESCs.