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. Author manuscript; available in PMC: 2013 Nov 29.
Published in final edited form as: Gene. 2004 Oct 27;341:10.1016/j.gene.2004.06.043. doi: 10.1016/j.gene.2004.06.043

Fig. 1.

Fig. 1

Cloning of full-length LRAT mRNA from human liver. (A, B) Identification of the 5′-end of the LRAT mRNA. Ethidium bromide-stained agarose gel after electrophoresis of the PCR products (A) and nested PCR products (B) of the 5′-end of LRAT mRNA as cloned by the GeneRacer kit (see text for details). M, low-mass DNA marker. (C) Amplification of full-length LRAT mRNA from human liver. Ethidium bromide-stained agarose gel of the LRAT RT-PCR product amplified from human liver poly[A]+ RNA. Either of two sense primers (primers 10 and 11) with an antisense primer (primer 12) was designed from the 5′- and 3′-ends of the LRAT mRNA (lane 3, primer pair 10 and 12; lane 4, primer pair 11 and 12, see Table 1 for primer identification), and used for RT-PCR as described in Materials and methods. The LRAT amplicons from human liver had the expected size of about 4.8–4.9 kb. Lane 1, negative control; lane 2, high-mass DNA marker.