Table 1. Primers used for human liver LRAT cDNA cloning and analysis.
No. | Primer name | Region covered in mRNA | Primer sequence (5′→3′) |
---|---|---|---|
1 | HLT22-S | 832–855 | agatatggcaccccgatcagtccc |
2 | HLT23-A | 919–942 | agacgccaatcccaagactgctga |
3 | LT1-A | 496–515 | atgccatagtgggtcaggtg |
4 | LT2-A | 953–972 | agtgtatgataccaagcccg |
5 | HLT41-S | 2643–2667 | aaagcaaccactatgagaactaccg |
6 | HLT42-S | 2829–2850 | tgaagacccctcattaaaagcc |
7 | HLT45-S | 3703–3722 | ttctctgggcttcatccatt |
8 | HLT95-S | 104–123 | cttatccgtctcattcccca |
9 | HLT96-A | 594–613 | gcttgttggagaccaccttc |
10 | HLT105-S | 102–123 | tccttatccgtctcattcccca |
11 | HLT107-S | 45–66 | ctccttctccggctgcttgtag |
12 | HLT104-A | 4840–4861 | aacaaggacttttgccattgcc |
13 | HLT66-A | 446–469 | ctcggtggaaagagctggtttcat |
14 | HLT65-A | 505–528 | gtctcctaggtagatgccatagtg |