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FKBP51 expression increases during the process of 3T3-L1 preadipocyte differentiation. (A) 3T3-L1 cells were differentiated for the indicated periods of time, cell lysates were obtained and proteins were separated by SDS-PAGE. Immunoblotting was performed with the indicated antibodies. (B) mRNA was isolated from 3T3-L1 at the indicated times post-induction of adipogenesis and analyzed as described in Materials and Methods. Similar results were obtained in three different experiments.
