Fig. 6.

The mechanism of action for TRIO, CHKA and BMX lies between the AT₁R and EGFR. (A) Our hypothesis is that TRIO, CHKA and BMX lie between the activated AT₁R and EGFR and thus modulate the AT₁R–EGFR transactivation response. A proposed model for this theory is illustrated. (B) HMEC-LST-AT₁R cells were transfected with siGFP, siTRIO, siCHKA or siBMX SMARTpool siRNAs for 72 hours and stimulated with either AngII (100 nM) or EGF ligand (0.5 ng/ml) for 10 minutes. Knockdown of TRIO (B), BMX (C) or CHKA (D) did not alter the response of the cells to 0.5 ng/ml EGF ligand. Data are representative of three independent experiments.