Fig. 2.

Baseline muscle characteristics in 2-month-old WT and NebΔSH3 muscle fibers. Fiber type distribution as determined by relative amounts of type 1, 2A, 2X and 2B myosin heavy chain isoforms in TA (A), EDL (B) and soleus (C) muscle from WT and NebΔSH3 muscle. (D) Fiber cross sectional area (CSA) in TA, EDL and soleus muscle. n = 6 (A–D). (E) TEM of EDL and diaphragm muscle from 1-year-old WT and NebΔSH3 mice. Scale bar: 1 µm. (F) Hematoxylin and Eosin staining 4, 7, 14 and 21 days after injection of cardiotoxin into the TA of 2-month-old WT and NebΔSH3 mice. Scale bar: 50 µm.