Fig. 4.

The absence of the nebulin SH3 domain does not affect the localization of its interaction partners. (A) Immunostaining of cryosectioned WT and NebΔSH3 TA muscle with antibodies against myopalladin (MYPN), palladin (PALLD), zyxin and N-WASP, showing colocalization with α-actinin or desmin in the Z-line. (B) Immunostaining of cryosectioned WT and NebΔSH3 TA muscle during regeneration 14 days after injection of cardiotoxin, showing targeting of myopalladin and N-WASP to the Z-line in both WT and NebΔSH3 muscle. (C) Immunostaining for N-WASP of cryosectioned TA muscle from WT and NebΔSH3 mice after 48 hours of starvation and at different time points following administration of IGF-1 to 48-hour prefasted mice. All scale bars: 5 µm. (D) Top: western blot analysis for myopalladin and N-WASP on cytosolic and cytoskeletal fractions of TA muscle from WT and NebΔSH3 mice. Bottom: western blot analysis for N-WASP on fractionated TA muscle from WT and NebΔSH3 mice at basal conditions, after 48 hours of starvation, and 2 hours after administration of IGF-1 to prefasted mice.