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. 2013 Dec 1;126(23):5490–5499. doi: 10.1242/jcs.138438

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — EGFP–VMAT1 localises to WPBs, and results in the appearance of WPB 5-HT immunoreactivity and current spikes during endothelial cell stimulation. (A) HUVECs expressing EGFP–VMAT1 and immunolabelled with specific antibodies to VWF (blue, rabbit Ab), EGFP (green) and 5-HT (red). Scale bar: 20 µm. Regions indicated by white boxes are shown in grey scale to the right at an expanded scale. (B) Simultaneous recording of fura-2 fluorescence ratio (top trace) and electrode current (bottom trace) from a single proregion–EGFP- and mCherry–VMAT1-expressing HUVECs following mechanical stimulation with the electrode.