Fig. 6.

Glycosylation of consecutive gap-1 sequons. (A) The sequences of HpΔ14 derivatives that have two to five closely spaced sites. (B–D) HeLa cells expressing Hp glycosylation site mutants were pulsed for 4 minutes and chased for 20 minutes. (C) Limited digestion of anti-DDK immunoprecipitates with endoglycosidase H (EH, 0–15 minutes). (D) HeLa cells were treated with negative control (NC) siRNA or siRNAs specific for STT3A or STT3B as indicated for 48 hours prior to transfection with Hp expression constructs. Quantified values below gel lanes are for the displayed image that is representative of two or more experiments.