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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Carbohydr Polym. 2013 Sep 2;99:10.1016/j.carbpol.2013.08.082. doi: 10.1016/j.carbpol.2013.08.082

Fig. 3.

Fig. 3

OGT mediates LPS-induced O-GlcNAcylation of NF-κB/p65. Western blotting of indicated proteins of either the whole cell lysates (used to assess the protein input as a loading control) or WGA-enriched O-GlcNAc modified proteins, isolated respectively from endothelial cells being treated (A) either with vehicle (culture medium) or ST045849 (10 μM), a potent OGT inhibitor, for 1 h followed by LPS (100 ng/ml) challenge for 1 h (in BAEC) and (B) either with control or OGT siRNA for 48h followed by LPS (100 ng/ml) challenge for 1 h (in EA.hy926). Data are expressed as means ± SEM (n=3). ##P<0.01 compared to the vehicle-treated control group; *P<0.05, **P<0.01 compared to the LPS-only group or the group of LPS-only with control siRNA treatment.