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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Carbohydr Polym. 2013 Sep 2;99:10.1016/j.carbpol.2013.08.082. doi: 10.1016/j.carbpol.2013.08.082

Fig. 4.

Fig. 4

OGT mediates LPS-induced NF-κB/p65 nucleus entry and activation. (A) Western blotting of the nucleus fraction proteins from EA.hy926 treated either with control or OGT siRNA for 48h followed by LPS (100 ng/ml) challenge for 1 h. (B) mRNA levels of inflammatory cytokines of EA.hy926 either treated with vehicle (culture medium) or ST045849 (10 μM), a potent OGT inhibitor, for 1 h, followed by incubation with LPS (100 ng/ml) for 4 h. The mRNA levels IL-1β, MCP-1, and E-selectin were determined by using RT-PCR as described in detail in Materials and Methods (Inflammatory cytokine gene expression analysis). Data are expressed as means ± SEM (n=3). #P<0.05, ##P<0.01 compared to the vehicle-treated control group; *P<0.05, **P<0.01 compared to the LPS-only group or the group of LPS-only with control siRNA treatment.