Table 1.
Comparison of development of ICSI oocytes and embryos after different electrical stimuli
| Treatment | No. of oocytes | No. of oocytes survived (%) | No. of pronuclei formation (%)* | No. of embryos cleaved (%)** | No. of embryos blastocysts (%)+ | No. of EGFP-positive blastocyst (%)++ |
|---|---|---|---|---|---|---|
| Without activation | ||||||
| ICSI | 235 | 205 (87)a | 67 (33)b | 60 (29)d | 11 (5)c | 1 (10)b |
| Sham | 40 | 40 (100)a | 0 (0)d | 0 (0)f | 0 (0)d | NA |
| One pulse before ICSI | ||||||
| ICSI | 139 | 126 (90)a | 88 (70)a | 75 (60)b | 43 (34)a | 10 (23)a |
| Sham | 45 | 42 (93)a | 6 (14)c | 5 (12)e | 0 (0)d | NA |
| Two pulses before ICSI | ||||||
| ICSI | 86 | 69 (80)a | 46 (67)a | 43 (62)b | 26 (37)a | 3 (12)b |
| Sham | 48 | 40 (83)a | 29 (73)a | 28 (70)a | 16 (40)a | NA |
| One pulse after ICSI | ||||||
| ICSI | 46 | 22 (48)b | 15 (68)a | 13 (59)b | 4 (18)b | 0 (0)c |
| One pulse before and after ICSI | ||||||
| ICSI | 38 | 18 (47)b | 10 (56)a | 8 (44)c | 2 (11)b | 0 (0)c |
Different letter superscripts in the same column indicate a significant difference (p < 0.05)
NA not available
* Percentage of embryos with pronuclei/surviving oocytes
** Percentage cleaved/surviving oocytes
+ Percentage of blastocysts/surviving oocytes
++ Percentage of EGFP-positive embryos/blastocyst embryos