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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Anal Chem. 2013 Oct 28;85(22):10.1021/ac402871k. doi: 10.1021/ac402871k

Figure 4.

Figure 4

Modeled and actual EGFP release from an affinity column as a function of temperature, triglycine concentration, and time. A GFP-STEPL fusion protein was expressed and washed on an affinity column. The column was then treated with 0mM (asterisk), 25mM (star), 100mM (diamond), 200mM (square), or 5mM (circle) triglycine and 100mM Ca2+. GFP release was monitored as a function of time. Protein release experiments were conducted at 6°C (top), 25°C (middle), and 37°C (bottom). All data was fit using a kinetic model of EGFP cleavage that takes into account both triglycine-dependent and triglycine-independent pathways. Modeled GFP release (lines) has been superimposed onto the recorded data (symbols).