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Functional evaluation of the Her2 affibody-HiLyte Fluor™ 750 conjugate. (A) Her2/neu positive and negative cells were incubated with Her2/neu-targeted affibodies that were conjugated to HiLyte Fluor™ 750 (red) using the STEPL system. Cells were also stained with Hoechst 33342 (nuclear stain, blue). (B) In-cell western quantification of HiLyte Fluor™ 750 fluorescence.
