Figure 2.

FKN is a direct primary functional target of miR-195. A: FKN possesses binding sites for miR-195 in its 3′-UTRs. B: Luciferase assays indicated that miR-195 directly lowered the expression of FKN in the ectopic ESC. Relative luciferase values were normalized to co-transfection with the psiCHECK-2-control. Data represent the mean±SD from 2 separate determinations performed in triplicate. **P<0.01. C: Western blot shows the endogenous expression levels of FKN after transfection with miR-195 or FKN siRNA in ESC. β-actin served as an internal control.