Figure 3.

MiR-195 inhibits proliferation and growth and induces apoptosis of ESCs by targeting FKN. A: BrdU proliferation assays were used to analyze proliferation of ESCs. Normal, eutopic and ectopic ESCs [1×10⁴ cell/well] from women with endometriosis were treated, respectively, with recombinant human FKN (100 ng/ml) for 24 h, transfected with miR-195 mimics (50 pmol/well), or FKN-siRNA (50 pmol/well) or vehicle as controls for another 24 h. B: Cell number counts were used to analyze growth of ESCs. C: Annexin V-FITC apoptosis detection assays were used to analyze apoptosis of normal ESCs. D: Annexin V-FITC apoptosis detection assays were used to analyze apoptosis of eutopic ESCs associated with endometriosis. E: Annexin V-FITC apoptosis detection assays were used to analyze apoptosis of ectopic ESCs. Results were highly reproducible in three independent experiments. Error bars depict the standard error of the mean. *P<0.05, **P<0.01 compared with the vehicle control. #P<0.05, ##P<0.01 compared with the miR-195 treatment.