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Schematic drawing of the hanging drop model. A cell suspension containing 50,000 cells/ml, supplements (e.g. TGF-β) and 20% Methocel was dropped onto a petri dish cover. The cover was then carefully inverted and set on the respective petri dish filled with PBS. The hanging drops were incubated for 1, 2, 4 or 7 d in the CO2-incubator.
