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. Author manuscript; available in PMC: 2013 Nov 29.
Published in final edited form as: Neurochem Res. 2004 Jun;29(6):1065–1081. doi: 10.1023/b:nere.0000023594.21352.17

Fig. 4.

Fig. 4

Spot matching and quantitation with different two-dimensional electrophoresis approaches. (A) Spot matching is the process of determining which spot is the same across multiple gels. Two-dimensional DIGE aids matching by using an internal control channel present on each gel and fewer gels in total. (B) Quantitation of traditional 2-DE uses densitometry of matched spots. 2-DIGE improves quantitation by assessing abundance in relation to a normalization pool on each gel, thereby normalizing for gel to gel technical differences.