[Table/Fig-2]:

Amplification products of the six common Apo E alleles with modified ARMS on agarose gel electrophoresis. Each sample was analyzed by loading two different PCR reaction mixtures. Mixture A contains the Arg112 (451 bp) and Arg158 (588 bp) specific ARMS primers. Similarly, mixture B contains the specific ARMS primers Cys112 (451 bp) and Cys158 (588 bp). Both mixtures contain the same common ARMS primer as well as a set of internal control primers that amplify a 360-bp segment of the α1-antitrypsin gene.

Lanes 1 and 2, negative DNA control;

Lane 3 and 4, E3E2(Arg112 positive, Arg158 negative; Cys112 positive, Cys158 positive);

Lane 5 and 6, E4E2 (Arg112 positive, Arg158 positive; Cys112 positive, Cys158 positive);

Lane 7 and 8, E4E3(Arg112 positive, Arg158 positive; Cys112 negative, Cys158 positive);

Lane 9 and 10, E4E4(Arg112 positive, Arg158 positive; Cys112 negative, Cys158 negative);

Lane 11 and 12, E3E3 (Arg112 positive, Arg158 negative; Cys112 negative, Cys158 positive);

Lane 13 and 14, E2E2 (Arg112 negative, Arg158 negative; Cys112 positive, Cys158 positive);

Lane 15, 100 bp DNA marker