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. Author manuscript; available in PMC: 2013 Nov 29.
Published in final edited form as: Chem Res Toxicol. 2010 Jun 21;23(6):10.1021/tx900367p. doi: 10.1021/tx900367p

Figure 3. LC-MS/MS chromatograms and CID spectra showing the presence of the GSH conjugate of 5-hydroxythalidomide formed in the presence of 5 mM GSH.

Figure 3

(A) Extracted ion chromatogram of the product ion with m/z 451 of the 5-hydroxythalidomide-GSH conjugate. (B) MS/MS spectrum of the 5-hydroxythalidomide-GSH conjugate (MH+ 580). (C) Total ion chromatogram of the CID of the m/z 451 product ion. (D) MS3 spectrum of the 5-hydroxythalidomide-GSH conjugate (m/z 580@30, 451@40). 5-hydroxythalidomide (100 µM) was incubated with the P450 3A4 bicistronic membranes (1 µM) in the presence of GSH (5 mM) and an NADPH-generating system for 3 h.