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. Author manuscript; available in PMC: 2013 Nov 29.
Published in final edited form as: Chem Res Toxicol. 2010 Jun 21;23(6):10.1021/tx900367p. doi: 10.1021/tx900367p

Figure 5. LC-MS/MS chromatograms showing the presence and relative yield of the GSH conjugate of 5-hydroxythalidomide formed from the (R)- and (S)-thalidomide enantiomers.

Figure 5

(A) (R)-thalidomide; (B) (S)-thalidomide. (R)- or (S)-5-hydroxythalidomide (100 µM) was incubated with the P450 3A4 bicistronic membranes (1 µM P450) in the presence of GSH (5 mM) and an NADPH-generating system for 60 min. Extracted ion chromatograms showing the m/z 580→451 transition are shown. MA: mass area.