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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Drug Deliv Transl Res. 2013 Oct 1;3(6):10.1007/s13346-013-0178-3. doi: 10.1007/s13346-013-0178-3

Figure 6.

Figure 6

Top row: Raman images of single SKOV-3 cells incubated with EGFR-targeted PLGA-PEG-PCL nanoparticles loaded with D11-C6-ceramide, over enumerated time periods. Single cells were imaged using a confocal Raman micro-spectral system and analyzed using VCA. Pseudo-colored images represent: cell body and nuclei – blue; membrane-rich organelles and vesicles – green; D11-C6-ceramide – red. Bottom row: corresponding cells counterstained after Raman data acquisition with Mitotracker Green. Same microscope setup was used to analyze fluorescence stain, at the same focal plane.