Fig. 2. Schematic diagram of mutant Pax5 proteins. (A) Domain structure of Pax5 and extent of amino acid deletion in the different mutant proteins. (B) The octapeptide sequence is shown together with the Y179E mutation and the ΔOP and Δ9A deletions. (C) Conservation of the octapeptide motif. The octapeptide sequences of mouse (m), zebrafish (zf) and Drosophila (d) members of the Pax2/5/8 family are aligned with the corresponding Engrailed homology region 1 (eh1) of the homeodomain transcription factors En2 and Goosecoid (Gsc). Identical amino acids are highlighted by black overlay. For sequences, see Smith and Jaynes (1996), Czerny et al. (1997) and Pfeffer et al. (1998).