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Expression of gro-α mRNA and protein after treatment with gro-α siRNA-loaded NPs. (A) Expression of gro-α mRNA as determined by qRT-PCR. (B) Expression of gro-α protein secreted in cell supernatants as determined by ELISA. ES-2 cells were treated with different nanoparticle complexes for 4 h. Gro-α mRNA and gro-α protein secreted in cell supernatants were quantified 24 h or 48 h after treatment.
