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. 2013 Nov 4;3:66. doi: 10.1186/2191-0855-3-66

Table 2.

Percentage (mean ± S.D.) of E. coli PHL644 pSTB7 cells that were alive determined using flow cytometry during biotransformations performed with planktonic cells or biofilms

Reaction conditions
Cell type and time of sampling
Planktonic
Planktonic
Biofilm
Biofilm
  2 hours 24 hours 2 hours 24 hours
Reaction Buffer, 5% DMSO
99.52 ± 0.14
99.32 ± 0.40
95.73 ± 2.98
92.34 ± 0.10
Reaction Buffer, 5% DMSO, 2 mM 5-fluoroindole
99.38 ± 0.60
99.24 ± 0.80
96.44 ± 1.51
90.73 ± 0.35
Reaction Buffer, 5% DMSO, 2 mM 5-chloroindole
99.27 ± 0.33
99.33 ± 0.20
95.98 ± 2.64
91.69 ± 3.09
Reaction Buffer, 5% DMSO, 2 mM 5-bromoindole 99.50 ± 0.18 99.33 ± 0.20 96.15 ± 1.94 91.17 ± 2.19