Table 1.

Analysis of monosaccharides released after enzymatic hydrolysis of leaves from transgenic and mutant plants

	Ara a	Gal a	Glc a	Xyl a
WT	0.11 ± 0.05	0.24 ± 0.04	0.99 ± 0.57	0.04 ± 0.02
pme3	0.11 ± 0.00	0.28 ± 0.03	2.18 ± 0.32*	0.05 ± 0.01
qua2-1	0.14 ± 0.01	0.21 ± 0.02	1.56. ± 0.05*	0.03 ± 0.0
PMEI	0.14 ± 0.01	0.30 ± 0.06	2.22 ± 0.07*	0.04 ± 0.01
PG	0.13 ± 0.00	0.21 ± 0.01	1.56 ± 0.09*	0.05 ± 0.01

^anmoles per μg of total sugar released after 24 hours of hydrolysis from WT (Col-0), pme3, qua2-1, PMEI, and PG plants, quantified using HPAEC-PAD. Values are expressed as nmoles of each sugar per μg of total sugars (TS) in the tissues. Results are means ± SD of three biological replicates. Asterisk (*) indicates statistically significant differences with respect to the WT according to Student’s t-test (P <0.05). The experiments were repeated at least three times with similar results. Ara, arabinose; Col-0, Columbia-0; Gal, galactose; Glc, glucose; HPAEC-PAD, high-performance anion-exchange chromatography with pulsed amperometric detection; PG, polygalacturonase; pme3, pectin methylesterase 3; PMEI, pectin methylesterase inhibitor; qua2-1, quasimodo2-1; WT, wild type; Xyl, xylose.