Figure 5.

Gibbs free energy of exchange (ΔG_HX) for residues of wild type Im7 (grey) and Im7H3M3 (blue) measured at 10°C, in 50 mM phosphate buffer, pH 7, and 0.4M Na₂SO₄. The dashed lines represent the ΔG⁰_UI (14.8 kJ/mol for Im7H3M3 and 12.4 kJ/mol for Im7) and the dotted lines represent the ΔG⁰_UN (23.3 kJ/mol for Im7H3M3 and 25.6 kJ/mol for Im7).²⁴ Secondary structure elements are illustrated at the top of the figure, with the extended helix III on the mutant Im7H3M3 displayed in red. The ΔG_HX values for Im7 were taken from Gorski et al.¹⁷ [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com.]