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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: Sci Signal. 2013 Jun 18;6(280):10.1126/scisignal.2003411. doi: 10.1126/scisignal.2003411

Fig. 7.

Fig. 7

PPM1A binds to the C-terminal domain of MAN1. 1H-15N 2D NMR spectra were recorded on 1:0 and 1:1 ratio samples of a 15N-labeled protein and its unlabelled partner. (A) Representative HSQC spectrum of 15N-labeled fragment of MAN1 from amino acid 1 to amino acid 471 (MAN1N) with (red) or without (blue) added PPM1A. N=2 independently generated replicates. (B) Representative HSQC spectrum of 15N-labeled fragment of MAN1 from amino acid 658 to amino acid 911 (MAN1C) with (red) or without (blue) added PPM1A. (C) Representative TROSY spectrum of 15N-labeled PPM1A with (red) or without (blue) addition of the MAN1 fragment from amino acid 1 to amino acid 471 (MAN1N). N=2 independently generated replicates. (D) Representative TROSY spectrum of 15N-labeled PPM1A with (red) or without (blue) addition of the MAN1 fragment from amino acid 658 to amino acid 911 (MAN1C). N=2 independently generated replicates. (E) Representative TROSY spectrum of 15N-labeled PPM1A with (red) or without (blue) addition of the MAN1 fragment from amino acid 755 to amino acid 911 (MAN1Luhm). N=2 independently generated replicates.