Fig. 1. (A) Colony screen for the detection of gid3-1-complementing genomic DNA fragments. Cells were transferred on nitrocellulose sheets as described in Materials and methods and probed with antibodies against FBPase. Further details are given in the text. gid mutant cells appear significantly darker than wild-type cells (arrowheads) due to their higher FBPase level. (B) Complementation of gid3-1 cells was verified in immunoblots following FBPase immunogenic material after retransformation of the plasmid, isolated from the clone shown in (A). The complementing plasmid re-introduces the FBPase degradation phenotype. (C) Subcloning identifies UBC8 as the complementing ORF.