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. 2013 Nov 29;8(11):e77972. doi: 10.1371/journal.pone.0077972

Figure 2. HIV-1 Tat colocalizes with APP in U-87 MG cells.

Figure 2

Fluorescence microscopy images of Tat-transfected U-87 MG cells immunostained with anti-Tat and anti-APP (A8717) antibodies are shown. U-87 MG cells were transfected with the wild-type Tat construct and incubated for 16 h. The cells were fixed and stained with anti-Tat or anti-APP antibodies followed by FITC-conjugated anti-mouse or rhodamine-conjugated anti-rabbit antibodies, respectively. Nuclear (A) and nuclear plus cytosolic (B and C) localization of Tat is shown. Scale bar = 10 µm.