Fig. 7. Ufd1/Npl4 does not function as a p97 co-factor in Golgi membrane fusion. (A) MGFs isolated through a 0.5 M sucrose cushion were incubated with p97/p47, p97, p47, p97/Ufd1/Npl4, p97/Ufd1, Npl4 or Ufd1 for 60 min at 37°C. Incubations were processed for EM, and the percentage of membrane in cisternae determined. Results are presented as the percentage cisternal regrowth, where 0% represents starting MGFs and 100% represents the effect of p97/p47. Values represent means ± SEM (n = 3). (B) MGFs were incubated as in (A) with p97/p47 in the presence of increasing amounts of Ufd1 alone or Ufd1/Npl4 (1, 5, 10 and 20 molar excess over p47).