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. 2013 Nov 29;8(11):e81203. doi: 10.1371/journal.pone.0081203

Figure 6. MicroRNA 126 (miR-126) inhibits cell proliferation, migration and invasion of colorectal cancer cells.

Figure 6

(A) HT-29 cells were transfected with various amounts of miR-126 mimic or negative control (NC) mimic and cell proliferation was analyzed using Cell Counting Kit-8. The miR-126 mimic showed dose-dependent effects on inhibiting cell proliferation after transfection for 48 h. (*P<0.05, compared to that of NC mimic). Data are presented as mean ± SEM of three independent experiments. HT-29 cells were transfected with 50 nM of miR-126 mimic or NC mimic (B) and HCT-116 cells were transfected with 100 nM of miR-126 inhibitor or NC inhibitor (C), migrated cells stained by 0.1% crystal violet were counted 48 h after transfection (×200). The number of migrated cells per field was obtained from at least three replicate wells and presented as mean ± SD (P<0.05) (bottom graph). (D, E) Invaded cells stained by 0.1% crystal violet were counted 48 h after transfection (×200). The number of invaded cells per field was obtained from at least three replicate wells and presented as mean ± SD (P<0.05) (bottom graph). *, indicates significant differences between HT-29-miR-126 mimic or HCT-116-miR-126 inhibitor group when compared to each NC group separately (P<0.05).