Table 1. Primers used for quantitative reverse transcription-polymerase chain reaction in this study.
Name | Direction | Primer (5′-3′) |
For microRNA qPCR | ||
Universal qPCR primer | Reverse | The One Step PrimeScript® miRNA cDNA Synthesis Kit (Takara) |
miR-126 | Forward | TCGTACCGTGAGTAATAATGCG |
U6 snRNA | Forward | CTCGCTTCGGCAGCACA |
For mRNA qPCR | ||
IRS-1 | Forward | AGTCCTAACCGCAACCAGAGT |
Reverse | CCTCAGCCACACATTCTCAA | |
Beta-actin | Forward | GGCGGCAACACCATGTACCCT |
Reverse | AGGGGCCGGACTCGTCATACT |