Table 4. Nucleotide and complementary protein-level selection assessment of Elapidae Nerve Growth Factors (NGF).
| Codon | Amino Aacid | M2aa | M8b | Propertyc | Magnituded | ASA |
|---|---|---|---|---|---|---|
| 57 | R | 4.241±0.515 | 3.894±0.510 | - | - | - |
| (0.994) | (0.998)* | |||||
| 72 | A | 4.260±0.453 | 3.899±0.493 | - | - | - |
| (0.999)** | (1.0)** | |||||
| 116 | T | 4.227±0.553 | 3.892±0.513 | Rα, αm | 6, 7 | - |
| (0.990)* | (0.997)** | |||||
| 123 | A | 4.243±0.509 | 3.895±0.506 | Rα, αm | 6, 7 | - |
| (0.994) | ( 0.998)* | |||||
| 125 | R | 4.132±0.769 | 3.857±0.601 | - | - | - |
| (0.961)* | (0.986)* | |||||
| 127 | N | 4.262±0.448 | 3.900±0.492 | Rα, αm | 6, 7 | - |
| (1.0)** | (1.0)** | |||||
| 133 | Q | 4.262±0.446 | 3.900±0.491 | Rα, αm | 6, 7 | - |
| (1.0)** | (1.0)** | |||||
| 144 | D | 4.258±0.459 | 3.899±0.495 | Rα, αm | 6, 7 | 69.5 |
| (0.999)** | (1.0)** | Exposed | ||||
| 150 | T | 4.252±0.481 | 3.897±0.500 | αm | 6 | 77.5 |
| (0.997)** | (0.999)** | Exposed | ||||
| 155 | R | 4.146±0.740 | 3.867±0.576 | αm | 6 | 87.0 |
| (0.965)* | (0.990)* | Exposed | ||||
| 158 | V | 4.247±0.498 | 3.896±0.504 | - | - | 76.9 |
| (0.995)** | (0.999)** | Exposed | ||||
| 163 | E | 4.257±0.464 | 3.898±0.496 | αm | 6 | 65.9 |
| (0.998)* | (0.999)** | Exposed | ||||
| 167 | L | 4.024±0.940 | 3.824±0.670 | αm | 6 | 65.0 |
| (0.929) | (0.976)* | Exposed | ||||
| 170 | E | 4.260±0.453 | 3.900±0.493 | - | - | 73.0 |
| (0.999)** | (1.0)** | Exposed | ||||
| 182 | R | 3.951±1.036 | 3.789±0.736 | - | - | 57.3 |
| (0.907) | (0.965)* | Exposed | ||||
| 207 | Q | 4.255±0.471 | 3.898±0.499 | Rα | 6 | 32.2 |
| (0.998)** | 0.999** | NA | ||||
| 211 | R | 4.260±0.455 | 3.899±0.493 | Rα | 6 | 53.1 |
| (0.999)* | (1.0)** | Exposed | ||||
| 219 | Q | 4.262±0.446 | 3.900±0.491 | Rα | 6 | 73.8 |
| (1.0)** | (1.0)** | Exposed | ||||
| 240 | D | 4.004±0.970 | 3.804±0.712 | Rα | 6 | 66.9 |
| (0.923) | (0.969)* | Exposed | ||||
| 167 | L | 4.024±0.940 | 3.824±0.670 | αm | 6 | 65.0 |
| (0.929) | (0.976)* | Exposed | ||||
| 170 | E | 4.260±0.453 | 3.900±0.493 | - | - | 73.0 |
| (0.999)** | (1.0)** | Exposed | ||||
| 182 | R | 3.951±1.036 | 3.789±0.736 | - | - | 57.3 |
| (0.907) | (0.965)* | Exposed | ||||
| 207 | Q | 4.255±0.471 | 3.898±0.499 | Rα | 6 | 32.2 |
| (0.998)** | 0.999** | NA | ||||
| 211 | R | 4.260±0.455 | 3.899±0.493 | Rα | 6 | 53.1 |
| (0.999)* | (1.0)** | Exposed | ||||
| 219 | Q | 4.262±0.446 | 3.900±0.491 | Rα | 6 | 73.8 |
| (1.0)** | (1.0)** | Exposed | ||||
| 240 | D | 4.004±0.970 | 3.804±0.712 | Rα | 6 | 66.9 |
| (0.923) | (0.969)* | Exposed |
Amino-acid property symbols used: Power to be in the middle of α-helix (αm), Solvent accessible reduction ratio (Rα)
PAML
a, b: Bayes Empirical Bayes (BEB) posterior probability and post-mean omega (indicated in brackets) for the sites detected as positively selected by the site models M2a and M8, respectively. Sites detected as positively selected at 0.95 and 0.99 posterior probability by the Bayes Empirical Bayes approach of M8 are represented by * and **, respectively.
TreeSAAP
c: amino acid property experiencing positive diversifying selection
d: magnitude of selection on the amino acid property
ASA: Accessible surface area.
Note: Codon sites with significant support from both nucleotide and protein-level selection analyses are highlighted in bold.