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. 2013 Nov 29;8(11):e81773. doi: 10.1371/journal.pone.0081773

Figure 5. Effect of rottlerin, LY294002, or rapamycin on Th2 and proinflammatory cytokine levels in OVA-inhaled mice.

Figure 5

(A, D) Western blotting of IL-4, IL-5, IL-13, TNF-α, and IL-1β in lung tissues. (B, E) Densitometric analyses are presented as the relative ratio of each molecule to actin. The relative ratio of each molecule in the lung tissues of SAL+SAL mice is arbitrarily presented as 1. (C, F) The levels of IL-4, IL-5, IL-13, TNF-α, and IL-1β were quantified by ELISA. The protein expression of these cytokines was measured at 48 h after the last challenge in mice. Results from four independent experiments with 5 mice/group are given as mean ± SEM. # p<0.05 vs SAL+SAL; *p<0.05 vs OVA+SAL. Saline-inhaled mice administered saline (SAL+SAL), OVA-inhaled mice administered saline (OVA+SAL), drug vehicle (OVA+VEH), rottlerin (OVA+ROT), LY294002 (OVA+LY), and rapamycin (OVA+RAPA).