Skip to main content
. 2013 Nov 29;8(11):e81773. doi: 10.1371/journal.pone.0081773

Figure 12. Effect of rottlerin or siRNA-PKC δ on phosphorylation of PKC δ and other signaling intermediates in TNF-α/IL-4-treated BEAS-2B cells.

Figure 12

Western blotting of the levels of phosphorylated (p-) PKC δ, PKC δ, and other signaling intermediates in TNF-α/IL-4-treated BEAS-2B cells. BEAS-2B cells were pretreated with rottlerin (ROT, 4 µM) for 6 h prior to challenge with TNF-α/IL-4 for the indicated time periods or transfected with siRNA targeting PKC δ (siRNA-PKC δ) or control siRNA (siRNA-Con) and then challenged with TNF-α/IL-4. Representative images with respective densitometry data (bar diagram) are shown. Results from four independent experiments with 5 mice/group are given as mean ± SEM. # p<0.05 vs the untreated group; *p<0.05 vs the group treated by TNF-α/IL-4.