Figure 1. Specificity of the NA-subtyping primers in Real-time PCRs.

Real-time PCRs were performed with N1, N2, N3 and N7-specific primer pairs and 10-fold serially diluted on- and off target NA plasmid templates (10 to 10⁸ copies), the ΔCt values for them were recorded in A, B, C and D part of Figure 1, respectively. "N1pr/N1pl" represents Real-time PCR with N1-plasmid template and N1-specific primers, and so on and so forth.