Table 1. Phenotypic data for Δgpi7 mutants.
| Strain 1 | Dry Biomass 2 , mg (SD) | Colony Diameter 3 , cm (SD) | Macroconidia Concentration 4 (SE) | Macroconidia Length 5 , μm (SD) | % Abberrant Macroconidia 6 (SE) | Perithecia (SE)7 |
|---|---|---|---|---|---|---|
| gpi7-11 | 156.7 (20.2) | 1.9 (0.1) | 1.13x105 (14.2x103) | 19.2 (5.5) | 10.1 (1.2) | 2.7 (0.2) |
| gpi7-92 | 131.7 (44.4) | 1.9 (0.1) | 1.22x105 (6.4x103) | 24.1 (7.1) | 8.4 (0.7) | 2.8 (0.3) |
| gpi7-11 + gpi7 | ND | 3.4 (0.06) | 0.96x105 (4.4x103) | 47.2 (8.9) | 1.2 (0.1) | ND |
| wt:hyg | ND | ND | ND | 54.4 (8.5) | 1.9 (0.3) | 2.8 (0.1) |
| wt | 136.7 (12.7) | 3.3 (0.12) | 0.91x105 (8.1x103) | 49.1 (11.0) | 1.8 (0.4) | 3.1 (0.3) |
– ‘gpi7-11’ and ‘gpi7-92’ are Δgpi7 strains; ‘gpi7-11 + gpi7’ is strain gpi7-11 complemented with a full length gpi7 coding sequence. ‘wt’ is strain PH-1. ‘wt:hyg’ is wt strain PH-1 expressing a hygromycin phosphotransferase cassette.
– Mean dry biomass recorded after 3 days growth in liquid YMA media. Three replicates were analyzed for each strain. SD = standard deviation.
– Mean colony diameter of strains after 3 days growth on solid V8 medium. Three replicates were analyzed for each strain.
– Mean macroconida concentration of liquid CMC cultures growing at room temperature after 5 days. Three replicates were analyzed for each strain. SE = standard error.
– Mean macroconidia length of strains grown in liquid CMC. A minimum of 50 macroconidia were measured for each strain.
– Mean percentage of macroconidia displaying a “fused” phenotype (see Fig. 5.3). Over 1000 macroconidia were analyzed over 3 replicates for each strain.
– Mean density of perithecia on carrot agar plates (no. perithecia mm-1). Three replicates were analyzed for each strain.
ND = not determined.