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. 2013 Nov 29;8(11):e82180. doi: 10.1371/journal.pone.0082180

Figure 1. GPR35 is expressed in mouse cortical astrocytes and its activation provokes a decrease of FRSK-induced cAMP production.

Figure 1

A) GPR35 transcripts were detected in cultured mouse astrocytes and in DRG after 10 days of in vitro cultures by RT-PCR. Ribosomal 18S RNA was amplified as an internal control. PCR products were analyzed by means of agarose gel electrophoresis. B) Concentration-response curve of KYNA on FRSK-activated cAMP formation in mouse astrocytic culture. Inset: zaprinast (1 µM), a different GPR35 agonist, induces a decrease of FRSK-activated cAMP formation. C) CID, prevents KYNA effects on FRSK-activated cAMP formation. D, E and F) GPR35 mRNA silencing reduces RNA expression and abolishes KYNA (10 µM) effects on FRSK-activated cAMP formation.